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A practical method for screening for beta-galactosidase secreting microbial colonies BJM
Barreto,Eriana S.; Silva,Daison O.; Passos,Flavia M. Lopes.
Microbial colonies were replicated on YNB® agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROL®) system. The pink color developed around the colonies was the result of the reaction of the glucose generated by the extracellular hydrolysis of lactose by beta-galactosidase, indicating secretion of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for beta-galactosidase secretion by microbial cells.
Tipo: Info:eu-repo/semantics/other Palavras-chave: BIOTROL®; Beta-galactosidase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100009
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Leveduras associadas a Spermologus rufus Boheman (Coleoptera: Curculionidae) Anais da SEB
Barreto,Marliton R.; Barreto,Eriana S.; Anjos,Norivaldo.
Larvae and adults of Spermologus rufus Boheman can cause severe damage to seeds of Brazilian pine, Araucaria angustifolia. In this study, yeasts were isolated from seeds of A. angustifolia and from the digestive tract of S. rufus to verify their similarity and determine the existence of feeding sites in order to elaborate an artificial diet for this insect. Pichia spp. were found in seeds and Pichia spp. and P. stipitis Pignal in the digestive tract of S. rufus. The low variability among observed microorganisms suggests a food preference, which can facilitate the elaboration of artificial diets.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Coleoptera; Curculionidae; Yeast; Pichia; Artificial diets; Brazilian pine.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000200017
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